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KMID : 0545120130230081084
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Journal of Microbiology and Biotechnology
2013 Volume.23 No. 8 p.1084 ~ p.1091
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Statistical Optimization of Medium Components for Milk-Clotting Enzyme Production by Bacillus amyloliquefaciens D4 Using Wheat Bran-an Agro-Industry Waste
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Weibing Zhang
Xiaoling He
Hongna Liu
Huiyuan Guo
Fazheng Ren
Weidong Gao
Pengcheng Wen
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Abstract
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In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett-Burman experimental design. Subsequently, an optimal medium was obtained using the Box-Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4·7H2O, 0.1 g/l of MgSO4·7H2O, 0.1 g/l of MnSO4·2H2O, 0.1 g/l of ZnSO4·7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett-Burman design and Box-Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.
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KEYWORD
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Optimization, Enzyme Production, Bacillus amyloliquefaciens D4, Response surface methodology
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